Journal: Aging Clinical and Experimental Research

Article Title: Effect of targeted intervention on C-terminal agrin fragment and its association with the components of sarcopenia: a scoping review

doi: 10.1007/s40520-023-02396-w

Figure Lengend Snippet: Association between levels of CAF and primary sarcopenia

Article Snippet: Scherbakov et al. 2016 Cohort study , Evaluation of C-terminal Agrin Fragment as a marker of muscle wasting in patients after acute stroke during early rehabilitation , To evaluate agrin as a marker of muscle wasting in patients with stroke , Studied 123 patients (age ranging from 42 to 98 years) with confirmed diagnosis of ischemic or hemorrhagic stroke , NR , Exclusion criteria for this observational study were acute and chronic inflammatory diseases, acute heart failure or myocardial infarction, liver cirrhosis, and dialysis, immune suppressive therapy, and history of cancer within the last 5 years , Stroke , NR , Venous blood samples were obtained under standardized conditions after overnight fasting and after 15 min of supine resting in a quiet and air conditioned room. Samples were centrifuged at 3500 rpm for 15 min (2000 × g), aliquoted and stored at − 80 °C until analysis Serum Commercially available enzyme-linked immunosorbent assay (ELISA) kit (NTCAF Elisa Kit; Neurotune, Schieren, Switzerland) , Body composition was assessed by bioelectrical impedance analysis (BIA) (QuadScan 4000, Bodystat Limited, UK) , Arm strength was analyzed using the handgrip dynamometer (Saehan Corporation, Korea) , NA , The relationship between variables was analyzed by linear and multiple regression analyses , CAF levels were elevated in stroke patients at admission (134.3 ± 52.3 pM) and showed incomplete recovery until discharge (118.2 ± 42.7 pM) compared to healthy controls (95.7 ± 31.8 pM, p < 0.001) Association between CAF levels and HGS ( r = 0.2, p < 0.05) of the non-paretic arm.

Techniques: Western Blot, Concentration Assay, Standard Deviation, Marker, Biomarker Assay, Enzyme-linked Immunosorbent Assay, Activity Assay, Sampling, Functional Assay

Journal: Aging Clinical and Experimental Research

Article Title: Effect of targeted intervention on C-terminal agrin fragment and its association with the components of sarcopenia: a scoping review

doi: 10.1007/s40520-023-02396-w

Figure Lengend Snippet: Effect of interventions (exercise/nutrition/hormonal) on the levels of the CAF

Article Snippet: Scherbakov et al. 2016 Cohort study , Evaluation of C-terminal Agrin Fragment as a marker of muscle wasting in patients after acute stroke during early rehabilitation , To evaluate agrin as a marker of muscle wasting in patients with stroke , Studied 123 patients (age ranging from 42 to 98 years) with confirmed diagnosis of ischemic or hemorrhagic stroke , NR , Exclusion criteria for this observational study were acute and chronic inflammatory diseases, acute heart failure or myocardial infarction, liver cirrhosis, and dialysis, immune suppressive therapy, and history of cancer within the last 5 years , Stroke , NR , Venous blood samples were obtained under standardized conditions after overnight fasting and after 15 min of supine resting in a quiet and air conditioned room. Samples were centrifuged at 3500 rpm for 15 min (2000 × g), aliquoted and stored at − 80 °C until analysis Serum Commercially available enzyme-linked immunosorbent assay (ELISA) kit (NTCAF Elisa Kit; Neurotune, Schieren, Switzerland) , Body composition was assessed by bioelectrical impedance analysis (BIA) (QuadScan 4000, Bodystat Limited, UK) , Arm strength was analyzed using the handgrip dynamometer (Saehan Corporation, Korea) , NA , The relationship between variables was analyzed by linear and multiple regression analyses , CAF levels were elevated in stroke patients at admission (134.3 ± 52.3 pM) and showed incomplete recovery until discharge (118.2 ± 42.7 pM) compared to healthy controls (95.7 ± 31.8 pM, p < 0.001) Association between CAF levels and HGS ( r = 0.2, p < 0.05) of the non-paretic arm.

Techniques: Marker, Concentration Assay, Activity Assay, Western Blot, Produced, Imaging, Enzyme-linked Immunosorbent Assay, Functional Assay, Modification